Journal: Pathobiology
Article Title: Keratin 24 Predicts Poor Prognosis in Breast Cancer
doi: 10.1159/000546022
Figure Lengend Snippet: Western blot and immunohistochemical expression of KRT24 in BC. Western blotting results for KRT24 expression in MCF7 and SKBR3 BC cell lines. a Red line is the housekeeping gene, while the green line is KRT24. b–f Morphological characteristics of KRT24 IHC in full-face BC tissue are shown. Normal mammary gland ductal cells show homogenous strong KRT24 staining; ×10 magnification ( b ). The reactivity of DCIS is heterogeneous and shows weak staining intensity; ×10 magnification ( c ) and ×40 magnification ( d ). Invasive cancer cells show homogenous strong KRT24 staining; ×10 magnification ( e ) and ×40 magnification ( f ). g–k KRT24 protein expression in BC TMA cores is shown. g and h show a strong cytoplasmic and membranous staining pattern (HC-HM) in cancer cells: ×10 magnification ( g ) and ×40 magnification ( h ). i demonstrates BC with moderate staining intensity in both the cytoplasm and membrane (MC-MM) at ×40 magnification. j and k show weak (WC-WM) to absent (AC-AM) staining intensity in both the cytoplasm and membrane: ×10 magnification ( j ) and ×40 magnification ( k ). DCIS, ductal carcinoma in situ.
Article Snippet: To validate the specificity of KRT24, both luminal A type (MCF7) and HER2-positive ER− (SKBR3) human BC cell lines (obtained from the American Type Culture Collection, Rockville, MD, USA) were used, as recommended by the manufacturer.
Techniques: Western Blot, Immunohistochemical staining, Expressing, Staining, Membrane, In Situ